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Title: | Effects on the quality of frozen-thawed alpaca (Lama pacos) semen using two different cryoprotectants and extenders | Authors: | Santiani, Alexei Huanca López, Wilfredo Sapana Valdivia, Rómulo Huanca Mamani, Teodosio Sepúlveda, Néstor Sánchez, Raúl |
Keywords: | Cryopreservation;Glycerol;Ethylene glycol;Extenders;Alpaca spermatozoa | Issue Date: | 1-Sep-2005 | Publisher: | Wolters Kluwer Medknow Publications | Source: | Santiani, A.; Huanca, W.; Sapana, R.; Huanca, T.; Sepúlveda, N.; & Sánchez, R. (2005). Effects on the quality of frozen‐thawed alpaca (Lama pacos) semen using two different cryoprotectants and extenders. Asian journal of Andrology, 7(3), 303-309. doi: 10.1111/j.1745-7262.2005.00021.x | Journal: | Asian Journal of Andrology | Abstract: | Aim: To evaluate two extenders and two cryoprotectant agents (CPA) for alpaca semen cryopreservation. Methods: Semen samples were obtained from four adult alpacas (Lama pacos) and frozen using extender I (TRIS, citrate, egg yolk and glucose) or extender II (skim milk, egg yolk and fructose), each containing either glycerol (G) or ethylene glycol (EG) as CPA. Consequently, four groups were formed: 1) extender I-G; 2) extender I-EG; 3) extender II-G; and 4) extender II-EG. Semen was diluted in a two-step process: for cooling to 5 °C (extenders without CPA), and for freezing (extenders with CPA). Viability and acrosome integrity were assessed using trypan blue and Giemsa stains. Results: When compared, the motility after thawing was higher (P < 0.05) in groups II-EG (20.0 % ± 6.7 %) and II-G (15.3 % ± 4.1 %) than that in groups I-G (4.0 % ± 1.1 %) and I-EG (1.0 % ± 1.4 %). Viable spermatozoa with intact acrosomes in groups II-EG (18.7 % ± 2.9 %) and II-G (12.7 % ± 5.9 %) were higher than that in groups I-G (5.7 % ± 1.5 %) and I-EG (4.0 % ± 1.0 %). Conclusion: The skim milk- and egg yolk-based extenders containing ethylene glycol or glycerol to freeze alpaca semen seems to promote the survival of more sperm cells with intact acrosomes than the other extenders. |
URI: | https://hdl.handle.net/20.500.12955/2172 https://pgc-snia.inia.gob.pe:8443/jspui/handle/pubitem/2491 |
ISSN: | 1008-682X | DOI: | 10.1111/j.1745-7262.2005.00021.x | Rights: | info:eu-repo/semantics/openAccess |
Appears in Collections: | Artículos científicos |
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Santiani_et-al_2005_cryopreservation_alpacas.pdf | Article (English) | 281,4 kB | Adobe PDF | View/Open |
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