Please use this identifier to cite or link to this item:
https://pgc-snia.inia.gob.pe:8443/jspui/handle/20.500.12955/2234
Title: | Does the addition of follicular fluid in the in vitro maturation medium increase the oocyte maturation and embryo production in alpacas? | Authors: | Contreras Huamani, Mijail Guillén Palomino, Crissthel Yverlin Laines Arcce, Irving Mitchell Silva Chaves, Maiana Magalhães Melo, Luciana De Figueirêdo Freitas, Vicente José |
Keywords: | Alpaca;Cumulus–oocyte complex;Oocyte maturation;Embryo production | Issue Date: | 7-Jun-2023 | Publisher: | Springer | Journal: | Tropical Animal Health and Production | Abstract: | In alpacas (Vicugna pacos), the high cost of in vitro embryo production is also a consequence of the use of several substances in the culture medium. In addition, embryo production rates in this species are still considered low. Thus, in attempt to reduce the cost and to improve the in vitro embryo production rates, this study evaluates the effect of adding follicular fluid (FF) in the in vitro maturation medium on oocyte maturation and subsequent embryo production. After ovary collection at the local slaughterhouse, the oocytes were recovered, selected, and allocated in experimental groups: standard maturation medium (G1) and simplified medium added by 10% FF (G2). The FF was acquired from follicles between 7- and 12-mm diameter. The cumulus cell expansion and the embryo production rates were analyzed by chi-square with p < 0.05. No differences (p > 0.05) were observed in maturation rate between G1 (66.36%) and G2 (63.12%) groups. Likewise, no significant difference (p > 0.05) was verified between G1 and G2 for morula (40.85 vs 38.45%), blastocyst (7.01 vs 6.93%), and total number of embryos (47.87 vs 45.38%). In conclusion, it was possible to simplify the medium used for in vitro maturation of alpaca oocytes resulting in embryo production rates similar to the standard medium. |
URI: | https://hdl.handle.net/20.500.12955/2234 https://pgc-snia.inia.gob.pe:8443/jspui/handle/20.500.12955/2234 |
ISSN: | 0049-4747 | DOI: | https://doi.org/10.1007/s11250-023-03646-9 | Rights: | info:eu-repo/semantics/restrictedAccess |
Appears in Collections: | Artículos científicos |
Show full item record
This item is licensed under a Creative Commons License